The Gen3 Kidney Organ-on-a-Chip (OOC) project aims to revolutionize preclinical drug testing through a modular, scalable, and automation-ready platform focused on complex human kidney model. Utilizing injection molding for cost-effective and reproducible manufacturing, the dual-channel microfluidic chip features cylindrical nephron tubule channels that replicate in vivo shear forces and support advanced biological modeling, including drug delivery and multi-cellular interactions. The system integrates seamlessly with high-throughput automation platforms and leverages AI/ML tools to analyze imaging and sensor data for real-time optimization. By addressing limitations in current OOC systems—such as high costs, limited scalability, and suboptimal physiological accuracy—this project delivers an innovative, sustainable, and cost-effective solution for pharmaceutical and academic research. With rising number of drugs discovered by AI driven systems, the need for a physiologically relevant, scalable system for drug screening becomes imminent. This project aligns with the EU Directive 2010/63 on the protection of animals used for scientific purposes, which enshrines the 3Rs (Replace, Reduce, Refine), and with the FDA’s modernization acts 2.0, 3.0.